mouse polyclonal antibody against eea1 (Santa Cruz Biotechnology)
Structured Review

Mouse Polyclonal Antibody Against Eea1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 708 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse polyclonal antibody against eea1/product/Santa Cruz Biotechnology
Average 96 stars, based on 708 article reviews
Images
1) Product Images from "Outer Membrane Vesicles From Probiotic and Commensal Escherichia coli Activate NOD1-Mediated Immune Responses in Intestinal Epithelial Cells"
Article Title: Outer Membrane Vesicles From Probiotic and Commensal Escherichia coli Activate NOD1-Mediated Immune Responses in Intestinal Epithelial Cells
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2018.00498
Figure Legend Snippet: NOD1 colocalizes with EcN and ECOR12 OMVs. HT-29 cells were incubated with EcN or ECOR12 OMVs (10 μg) for 1 h and analyzed using laser scanning confocal spectral microscope. NOD1 was stained using anti-NOD1 polyclonal antibody and Alexa Fluor 633-conjugated goat anti-rabbit IgG (red). OMVs were stained using anti- Escherichia coli LPS mouse monoclonal antibody followed by Alexa Fluor 488-conjugated goat anti-mouse IgG (green). Images are representative of three independent biological experiments. Colocalization of the red and green signals was confirmed by histogram analysis of the fluorescence intensities along the yellow lines. Analysis was performed by laser scanning confocal spectral microscope with 63x oil immersion objective lens, and images were captured with a Nikon color camera (8 bit). Scale bar: 10 μm.
Techniques Used: Incubation, Microscopy, Staining, Fluorescence
Figure Legend Snippet: Time-course analysis of NOD1 aggregation pattern in HT-29 cells in response to EcN or ECOR12 OMVs. HT-29 cells were incubated with EcN or ECOR12 OMVs (10 μg) for the indicated times and analyzed using laser scanning confocal spectral microscope. NOD1 was stained using anti-NOD1 polyclonal antibody and Alexa Fluor 633-conjugated goat anti-rabbit IgG. Images are representative of three independent biological experiments and are presented in color-code Fire. Analysis was performed as described for Figure . Scale bar: 10 μm.
Techniques Used: Incubation, Microscopy, Staining
Figure Legend Snippet: NOD1 colocalizes with EEA1-labeled endosomes in cells stimulated with EcN or ECOR 12 OMVs. HT-29 cells were incubated with EcN or ECOR12 OMVs (10 μg) for 1 h and colocalization of EEA1/NOD1 was analyzed using laser scanning confocal spectral microscope. NOD1 was stained using anti-NOD1 polyclonal antibody and Alexa Fluor 633-conjugated goat anti-rabbit IgG (red). Endosomes were detected with mouse polyclonal antibody against EEA1 and Alexa Fluor 488-conjugated goat anti-rabbit IgG (green). Images are representative of three independent biological experiments. Colocalization of the red and green signals was confirmed by histogram analysis of the fluorescence intensities along the yellow lines. Analysis was performed as described for Figure . Scale bar: 10 μm.
Techniques Used: Labeling, Incubation, Microscopy, Staining, Fluorescence